Read About Our Work and Products in the Literature

Alexander A. Cohen, Jennifer R. Keeffe ... Pamela J. Bjorkman

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Division of Biology and Biological Engineering, California Institute of Technology

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Immunization with mosaic-8b nanoparticles, which present eight SARS-like betacoronavirus (sarbecovirus) RBDs, elicits broader cross-reactive antibodies compared to SARS-CoV-2 RBD-only nanoparticles and offers protection against diverse sarbecoviruses. This study examined how prior COVID-19 vaccinations influence the immune response to mosaic-8b, finding that it induces more de novo antibodies targeting variant RBDs, supporting its potential to protect vaccinated or infected individuals from future SARS-CoV-2 variants and zoonotic sarbecoviruses.

Sai Nikhil Subraveti, Brian K. Wilson, Navid Bizmark, Jason Liu, Robert K. Prud'homme
 

Department of Chemical and Biological Engineering, Princeton University

 

A detailed protocol for synthesizing lipid nanoparticles (LNPs) using confined impinging jet (CIJ) mixer technologies, including a two-jet CIJ and a four-jet multi-inlet vortex mixer (µMIVM), is demonstrated. The CIJ mixers generate reproducible, turbulent micro-mixing environments, resulting in the production of monodisperse LNPs.

Stefanie Klisch, Dylan Gilbert, Emma Breaux, Aliyah Dalier, Sudipta Gupta, Bruno Jakobi, Gerald J. Schneider

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Department of Chemistry, Louisiana State University

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This work presents an automated extruder designed to enhance undergraduate research by providing a user-friendly system for preparing vesicles, such as liposomes, with precise size and polydispersity control. The project breaks down the development into software, hardware, and testing components, offering students hands-on experience with an accessible, simplified version of an industrial process, with all resources freely available online for further exploration and contribution.

Eric Wang, Alexander A. Cohen, Luis F. Caldera, Jennifer R. Keeffe, Annie V. Rorick, Yusuf M. Aida, Priyanthi N.P. Gnanapragasam, Pamela J. Bjorkman, Arup K. Chakraborty

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Institute for Medical Engineering and Science, Massachusetts Institute of Technology

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This study used computational methods to design nanoparticles displaying SARS-like betacoronavirus (sarbecovirus) receptor-binding domains (RBDs) with various mutations to enhance cross-reactive antibody responses. Among the designed antigens, mosaic-7COM elicited higher binding and neutralization titers in mice compared to other constructs, showing promise for protecting against highly mutated SARS-CoV-2 variants and zoonotic sarbecoviruses.